College of Natural and Health Sciences

Permanent URI for this community

https://hdl.handle.net/20.500.11868/717

Browse

Browsing College of Natural and Health Sciences by Issue Date

Filter results by year or month
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    The Effects of Chemical and Biological Gold Nanoparticles on Human Dermal Fibroblasts
    (The University of Tampa, 2019-05) Courtney, Samantha J.; Sneha Krishnamurthy
    Gold nanoparticles are found in consumer products that affect the skin such as cosmetics and anti-aging creams. This study explored the concentration dependent cytotoxic effects of both biogenic AuNPs and chemically synthesized citrate-capped AuNPs on human dermal fibroblast (HDF) cells. First, biogenic gold nanoparticles (AuNPs) were synthesized with 0.5 mM HAuCl4•3H2O using Camellia sinensis (black tea) as a reducing agent. Then, the crystallinity, size, and charge of the nanoparticles in suspension were analyzed by high-resolution transmission electron microscopy (HR-TEM), dynamic light scattering, and zeta potential analysis. Next, we analyzed the effects of AuNP concentration and exposure time on HDF cells. Immunofluorescence microscopy was performed using phalloidin and 4, 6 Diamidine-2-phenylindole dihydrochloride (DAPI) to analyze their effects on the actin filaments and nuclei of HDF cells. We assessed cell proliferation and viability using MTT assay, LDH assay, and flow cytometry. These results showed that the cytotoxic effects were similar for biological and chemical AuNPs, and at 72 hours of exposure, both AuNPs caused an increase in cell growth. Overall, there is no significant difference in the toxicity of biological and chemical AuNPs. In the future, we will analyze HDF cell gene expression after AuNP exposure with real-time PCR. Created as an independent study under the direction of Prof. Sneha Krishnamurthy.
  • Thumbnail Image
    Item
    Theoretical Adsorption of Lactic Acid Through Benchmark MOFs
    (The University of Tampa, 2019-06) Varghese, Abraham; Tony Pham
    The purpose of this project was to measure the adsorption capacity of benchmark MOFs in trapping lactic acid, a perilous biological substance. This project was completed under the direction of Prof. Tony Pham as part of a proposal for a URI grant.