An Analysis of Bafilomycin A1’s Effect on Theiler’s Murine Encephalomyelitis Virus in Baby Hamster Kidney Cells
Date
2023-04
Journal Title
Journal ISSN
Volume Title
Publisher
Keyhole Press, The University of Tampa
Abstract
Theiler’s murine encephalomyelitis virus (TMEV)
is an important picornavirus used to model the effects of
multiple sclerosis in mice. The purpose of this experiment
was to test the effect of the drug Bafilomycin A1 on TMEV
replication in BHK cells. We conducted our study on three
distinct samples, one sample was not treated with Bafilomycin
A1, the next was treated with Bafilomycin A1 before
infection with TMEV, and the last was treated with Bafilomycin
A1 after the cells were infected with TMEV. We
conducted four different tests on our samples. The first test
was a Plaque Assay; the results of this assay showed fewer
plaques in our pre-treatment sample, which indicated that
TMEV was inhibited when treated with Bafilomycin A1
before the cells were infected. The next test was a Western
Blot; the results we obtained from this test confirmed the
successful infection of the cells in each sample with TMEV.
We then conducted a TCID50 assay; the data validated the
results we obtained in our Plaque Assay. Our last test was
an immunofluorescence assay; the images confirmed and
showed that Bafilomycin A1 is more effective before infection
with TMEV. In the end, we concluded that Bafilomycin
A1 significantly inhibited TMEV when it was used preemptively
to treat cells. This paper was produced in Dr. Freundt’s
BIO 315 course.
Description
Recommended citation: Chacon, G., Bindra, A., & Potpora, S. (2023). An analysis of bafilomycin A1’s effect on theiler’s murine encephalomyelitis virus in baby hamster kidney cells. Q: Journal of Undergraduate Research & Inquiry, 4, 3-16. https://doi.org/10.48497/MTHC-RG58
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Article
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Keywords
Virus, Infection cycle, Cell treatment, Drug treatment
Citation
Chacon, G., Bindra, A., & Potpora, S. (2023). An analysis of bafilomycin A1’s effect on theiler’s murine encephalomyelitis virus in baby hamster kidney cells. Q: Journal of Undergraduate Research & Inquiry, 4, 3-16. https://doi.org/10.48497/MTHC-RG58