Phylogenetic Analysis of a Chlorovirus Isolated from New York State

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dc.contributor.advisorEric C. Freundt
dc.contributor.authorTara D. Temkar
dc.date.accessioned2018-02-21T19:57:26Z
dc.date.available2018-02-21T19:57:26Z
dc.date.issued2015
dc.descriptionRecommended Citation: Temkar, Tara D. “Phylogenetic Analysis of a Chlorovirus Isolated from New York State.” Acta Spartae, 2015. https://doi.org/10.48497/YVQQ-XW66.
dc.description.abstractChloroviruses are large icosahedral viruses that contain about 50 structural proteins, and typically infect certain green eukaryotic algae. Recently, chlorovirus sequences were isolated from throat swabs of healthy adults and were associated with cognitive impairment. Here, we analyzed a pond water sample from New York for the presence of chlorovirus. Cytopathic effects were observed in Chlorella heliozoae, strain SAG 3.83. A portion of the chlorovirus polymerase gene was amplified by the polymerase chain reaction using degenerate primers. The PCR product was bidirectionally sequenced. The sequence of the polymerase gene was compared to known chloroviruses and shown to be 98% identical to ATCV-1. The sequence of the environmental isolate was phylogenetically compared to related chloroviruses, and genetic relatedness to known viruses is presented.en_US
dc.identifier.citationTemkar, Tara D. “Phylogenetic Analysis of a Chlorovirus Isolated from New York State.” Acta Spartae, 2015. https://doi.org/10.48497/YVQQ-XW66.
dc.identifier.doihttps://doi.org/10.48497/yvqq-xw66
dc.identifier.urihttp://hdl.handle.net/20.500.11868/271
dc.language.isoen_USen_US
dc.publisherCollege of Natural and Health Sciences, The University of Tampaen_US
dc.subjectPhylogeneticen_US
dc.subjectDepartment of Biologyen_US
dc.subjectChlorovirusen_US
dc.subjectNew York Stateen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCESen_US
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Biologyen_US
dc.titlePhylogenetic Analysis of a Chlorovirus Isolated from New York Stateen_US
dc.typeArticleen_US

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Volume 1, No. 1, 2015