Browsing by Author "Michael L. Koren"
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Item Comparative Analysis of Triclosan Resistance in E. coli, S. aureus, and S. cerevisiae(College of Natural and Health Sciences, The University of Tampa, 2015) Michael L. Koren; Omari M. Richins; Eric C. FreundtThe introduction of antibiotics into everyday life has led to untreatable infections because some bacteria are resistant to nearly all forms of antibiotics. One antimicrobial, triclosan, has been used for over 30 years in an attempt to control microbe growth on commercial products. In this study, Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae were used as model organisms to demonstrate the effects of triclosan on gram-negative, gram-positive, and eukaryotic organisms, respectively. The organisms were grown in a 96-well plate that contained serial dilutions of triclosan. This study propagated triclosan resistance over several generations, which could provide insight into which group of organisms are more susceptible or resistant to triclosan. The results of this study showed that E. coli is sensitive to triclosan, whereas S. aureus and S. cerevisiae demonstrated higher levels of resistance.