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Transformation of a Mixed Probiotic Culture and Escherichia coli B with the Antibiotic Resistant Plasmid, pGLO

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dc.contributor.author Raven M. Wright, Haley L. Thompson and Eric Freundt
dc.date.accessioned 2018-03-06T20:44:51Z
dc.date.available 2018-03-06T20:44:51Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/20.500.11868/323
dc.description.abstract Probiotics are microorganisms residing in the gastrointestinal tract that have been shown to help with metabolic functions, immune responses, and in the prevention of pathogenic diseases. In this study, we sought to determine if the probiotic brand, Garden of Life, could acquire novel phenotypes through heat-shock transformation. We predicted that Garden of Life, along with Escherichia coli HB101 and Escherichia coli B would be able to grow on ampicillin enriched plates after transformation with the plasmid, pGLO, which contained the gene for beta-lactamase. During the study, all three bacterial cultures underwent heat shock transformation in the presence of pGLO. After transformation was complete, each new culture was plated on LB agar containing ampicillin and arabinose to determine whether antibiotic resistance and GFP expression were observed. The results showed that only Escherichia coli HB101 had successfully become antibiotic resistant. Our results demonstrate that the gram positive species in Garden of Life probiotics are not able to efficiently take up plasmid DNA using heat shock transformation and that the method appeared to have strain restrictions as well. en_US
dc.language.iso en_US en_US
dc.publisher College of Natural and Health Sciences, The University of Tampa en_US
dc.subject Escherichia coli B en_US
dc.subject pGLO en_US
dc.subject E-coli en_US
dc.subject Research Subject Categories::NATURAL SCIENCES en_US
dc.subject Research Subject Categories::NATURAL SCIENCES::Biology en_US
dc.subject Department of Biology en_US
dc.subject Probiotics en_US
dc.title Transformation of a Mixed Probiotic Culture and Escherichia coli B with the Antibiotic Resistant Plasmid, pGLO en_US
dc.type Article en_US


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