Phylogenetic Analysis of a Chlorovirus Isolated from New York State

Date

2015

Journal Title

Journal ISSN

Volume Title

Publisher

College of Natural and Health Sciences, The University of Tampa

Abstract

Chloroviruses are large icosahedral viruses that contain about 50 structural proteins, and typically infect certain green eukaryotic algae. Recently, chlorovirus sequences were isolated from throat swabs of healthy adults and were associated with cognitive impairment. Here, we analyzed a pond water sample from New York for the presence of chlorovirus. Cytopathic effects were observed in Chlorella heliozoae, strain SAG 3.83. A portion of the chlorovirus polymerase gene was amplified by the polymerase chain reaction using degenerate primers. The PCR product was bidirectionally sequenced. The sequence of the polymerase gene was compared to known chloroviruses and shown to be 98% identical to ATCV-1. The sequence of the environmental isolate was phylogenetically compared to related chloroviruses, and genetic relatedness to known viruses is presented.

Description

Recommended Citation: Temkar, Tara D. “Phylogenetic Analysis of a Chlorovirus Isolated from New York State.” Acta Spartae, 2015. https://doi.org/10.48497/YVQQ-XW66.

Keywords

Phylogenetic, Department of Biology, Chlorovirus, New York State, Research Subject Categories::NATURAL SCIENCES, Research Subject Categories::NATURAL SCIENCES::Biology

Citation

Temkar, Tara D. “Phylogenetic Analysis of a Chlorovirus Isolated from New York State.” Acta Spartae, 2015. https://doi.org/10.48497/YVQQ-XW66.